The course organised by Prof. Julio Fernandez will consist of ten days of lectures
and experimental work. The course will be focused on the study of protein mechanics
using atomic force microscopy (AFM). Recognized world experts will be teaching the
latest research on single-molecule biophysics with proteins.
The students will use a custom-made atomic force microscope directly imported from
Prof. Fernandez laboratory at Columbia University (New York). The students will setup,
perform and analyze experiments in the same manner that researchers do in their
innovative research in the Fernandez Laboratory. Several model proteins such as ubiquitin,
titin and thioredoxin will be employed.
The folding and unfolding force-dependent kinetics of single proteins as well as enzymatic
catalysis monitoring the rupture of single bonds will be studied in detail using the newly
developed force-clamp technique. The experiments will be complemented with practical
sessions of molecular dynamics simulations. The students will learn how to perform steered
molecular dynamics (SMD) as well as the application of molecular modeling to understand
protein dynamics and function.
The course represents a perfect opportunity for young students to introduce themselves in
the world of single-molecule with proteins. They will also have the opportunity to interact and
discuss top-notch scientific topics with the instructors in a friendly environment.